The effect of upstream wakes on heat transfer of endwall of blade was analyzed by heat transfer and numerical simulations. The wakes which affect the heat transfer of turbine blade occurred periodically due to the rotation of blade. The heat transfer is mainly affected by the rotation effects in high rotation cases, however, the heat transfer is mainly affected by the relative position of turbine blade and vane in low rotation cases. The wakes were simulated by cylindrical rods on the upstream of turbine blade passage in this paper. The non-rotating cylindrical rods were positioned on the upstream of blade to analyze the detailed effect of upstream wake. Cylindrical rods were positioned in four cases which are 0/4, 1/4, 2/4, 3/4 of turbine passage. We found out that the pressure and velocity distributions were differed with position of cylindrical rods. The occurrence of horseshoe vortex and passage vortex were affected by the developed wake. Secondary vortices were scattered when faced the upstream wakes. The heat transfer of endwall of turbine blade varies in different relative position cases. Therefore, we can assume that the wakes periodically affect the heat transfer of endwall of turbine blade.