Despite the extensive utilization of 3D printing, the direct printing of biomaterials has a limitation in terms of flexibility due to the original stiffness of material and limit of processing resolution. We overcame this limitation by incorporating dip-coating process with 3D printing of template. Briefly, 3D design was carried out on the basis of medical image data. According to the designed morphology, a sacrificial construct was 3D-printed using a water-soluble material. Thereafter, the 3D template was dip-coated with a solution of biomaterials. After the sacrificial template was removed by water, a micrometer-scale thin film tube could be obtained with retaining the 3D morphology. Owing to its thin geometry, the resulting structure exhibited a sufficient flexibility and suitable applicability to tubular scaffolds for tissue engineering. We demonstrated the feasibility by conducting animal surgery experiments and expect the universal applicability in other tubular tissue engineering such as vascular, airway, and abdominal organs.