A human in vitro cardiac tissue model would help to understand the cardiovascular disease and develop new strategies for cardiac diseases such as arrhythmias. In this research, we developed in vitro a three-dimensional (3D) human artificial heart tissue by populating synthetic filamentous matrices using multi-photon absorption by the femtosecond laser. With this advanced fabrication method. two-photon polymerization, the bio-inspired cardiac tissue scaffold had fabricated with a cohort of 3D filamentous matrices that precisely regulated the structural alignment of cardiac tissue. By varying the mechanical properties such as thickness and stiffness of filamentous structure, different level of cardiomyocytes contractility abnormality and susceptibility to drug-induced cardiotoxicity were measured under different physical environments.