Plants transport water from roots to leaves via xylem through transpiration, which is an evaporation process that occurs at the leaves. During transpiration, suction pressure can be generated by the porous structure of mesophyll cells in the leaves. Here, an artificial leaf mimicking structure using hydrogel, which has a nanoporous structure is fabricated. The cryogel method is used to develop a hierarchy structure on the nano- and microscale in the hydrogel media that is similar to the mesophyll cells and veins of a leaf, respectively. The theoretical model is analyzed to calculate the flow resistance in the artificial leaf, and compare the model with the experimental results. The experiment involves connecting a glass capillary tube at the bottom of the artificial leaf to observe the fluid velocity in the glass capillary tube generated by the suction pressure. The use of silicone oil as fluid instead of water in order to increase the flow resistance enables the measurement of negative pressure. The suction pressure of the artificial leaf is affected by several variables. Finally, by decreasing the pore size and increasing the wettability, the maximum suction pressure of the artificial leaf, -7.9 kPa is obtained.