Number of patients suffered with erectile dysfunction is increased in every year. The erectile dysfunction is caused by psychological and physical disorders such as diabetics and traumas. An erection is occurred by relaxation of smooth muscle in penis and trapping of blood inside of corpora cavernosa. Medications and injections are indicated for the treatment of erectile dysfunction. However, systemic symptoms like sudden vision loss, nausea, and irregular heartbeat are the major side effect of the medications. Also, patients with the erectile dysfunction have to directly penetrate own penis using syringes. Consequently, a topical ointment which has no systemic effect and pain accompanied by indication is desirable for the treatment of erectile dysfunction. Therefore, in this work, we developed microemulsions which contain nitric oxide and could be applied as an ointment.