In tissue engineering, to enhance the mechanical properties of three-dimensional scaffolds used for bone regeneration, it has been approached from the viewpoint of structure and chemistry. Meanwhile, in the structural field, the kagome structure has been known as one of structures having excellent relative strength. In this study, to enhance the mechanical properties of a biocompatible polymer scaffold for bone regeneration, we applied the three-dimensional kagome structure to a porous scaffold for bone regeneration. Firstly, kagome structure was designed by CATIA and converted into a STL file. Secondly, polycaprolactone (PCL) kagome scaffold was fabricated using 3D printer with a precision extruding deposition (PED) head. To observe morphology of fabricated PCL kagome scaffold, FE-SEM and laser microscope analysis were carried out. To measure mechanical property, UTM compressive test, three-point bending test, and numerical analysis were carried out and compared with conventional grid-structure scaffold. Moreover, Sarcoma osteogenic (Saos-2) cell experiments were used to evaluate the characteristics of in-vitro cell proliferation and rabbit calvarial defect model experiments were used to evaluate the bone regeneration characteristics of kagome scaffold.