Particles which have different material conditions and mechanical properties behave their special motions under the external conditions. Fe3O4 particles which have magnetic properties also have their own characteristics such as align through the solenoid-nozzle in external magnetic field. When the external magnetic field appears, Fe3O4 particles have been changed because of their magnetism compare with other non-magnetic particles like polystyrene particles. To optimize the solenoid-nozzle, change the magnet wire span size and connection method. The result show that span layer 1 and parallel connection generated high amp then span layer 5 and serial connection. We used 200nm fluorescent magnetic nano particles and 500nm fluorescent polystyrene particles which have different emission wavelength range to separate each image of particles under the one and two phase flow in the solenoid-nozzle. Both particles excited from 532nm laser system and their emission signals were imaged with two color fluorescent Particle Image Velocimetry(PIV). One EM-CCD camera with dichroic filter were used to capture images for separate phases. Instantaneous images for both magnetic and nonmagnetic nano particle was captured simultaneously.