There are many methods used to separation particles by size. However, in this study, we demonstrate that particles can be separated by using the difference in permittivity. Particles with a dielectric constant of 10, 40, and 70 differed in the speed of movement by dielectrophoresis in the same electric field, corresponding to the size of the CM factor. As a result, it was confirmed that the particles with a dielectric constant of 40 and 70 reach the wall surface at other positions in the same channel.