Stepwisely increasing or decreasing pressure, flow rate, shear stress and concentration have been broadly utilized for many applications. Generally, such applications are implemented with automated dynamic control systems that are expensive and difficult to use. Here, we present constant water-head-driven stepwise pressure generators which can stepwisely increase or decrease pressure without using any dynamic controllers. Its pressure increment and pressure-duration time can be independently tuned. With the connection of an additional microvalve, we applied the generators to the applications of stepwisely increasing or decreasing flow rate, shear stress, and concentration.