In this work, it studied the Self-Inflating Tire to prevent shortage/excessive tire pressure. Self-Inflating Tire is a technology that keeps the air pressure of the tire constant, which prevents accident risk and tire life reduction due to shortage/excessive air pressure. The shape optimization of the inner pumping tube of the tire was performed using ANSYS FSI(Fluid-Structure Interaction) technique. The tube optimization parameters were the outer diameter and thickness of the tube. The objective function was the inlet and outlet air pressure difference and outer diameter of the pumping tube. The optimization was performed using the central composite programming method, and a ANOVA table is used to verity the reliability of the regression model function estimated from the central composite table results. The shape optimization of the pumping tube was implemented from the verified ANOVA table.